The MAN2B2 Antibody (PAC056250) is a valuable tool for researching MAN2B2, an enzyme involved in the glycosylation process within the cell. This polyclonal antibody, generated from rabbits, is highly specific for human samples and has been validated for use in applications such as Western blotting. By binding to the MAN2B2 protein, this antibody allows for the detection and analysis of MAN2B2 expression in various cell types, making it ideal for studies in glycobiology and related fields.MAN2B2, also known as alpha-mannosidase 2B2, plays a crucial role in the breakdown of complex carbohydrates in the lysosome.
Dysregulation of MAN2B2 has been implicated in lysosomal storage disorders and other metabolic diseases, highlighting its importance in cellular function. Research into MAN2B2 activity can provide insights into disease mechanisms and potential therapeutic targets for conditions related to glycosylation abnormalities.By using the MAN2B2 Antibody in your research, you can further explore the functions and regulatory mechanisms of MAN2B2, contributing to the advancement of knowledge in glycobiology and potentially uncovering new avenues for therapeutic intervention in related disorders.
Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: MAN2B2 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114, 108 kDa. Observed band size: 114 kDa.
IHC image of PACO56250 diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: MAN2B2 antibody at 1.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114, 108 kDa. Observed band size: 135 kDa.
Epididymis-specific alpha-mannosidase (EC 3.2.1.24) (Mannosidase α class 2B member 2), MAN2B2, KIAA0935
UniProt Protein Function:
MAN2B2: Belongs to the glycosyl hydrolase 38 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.2.1.24; Secreted, signal peptide; Glycan Metabolism - other glycan degradation; Hydrolase; SecretedChromosomal Location of Human Ortholog: 4p16.1Molecular Function: zinc ion binding; carbohydrate binding; alpha-mannosidase activityBiological Process: mannose metabolic process
