The Magnetic Beads Conjugated Mouse Anti-DDDDK Tag Monoclonal Antibody (CABE037) is a powerful tool for researchers studying proteins tagged with the popular DDDDK sequence. This antibody, derived from mouse, is highly specific and sensitive, allowing for accurate detection and purification of tagged proteins in various biological samples.The DDDDK tag, also known as the FLAG tag, is commonly used in protein research for easy identification and isolation of recombinant proteins.
The conjugated magnetic beads enable efficient immunoprecipitation and protein purification processes, eliminating the need for labor-intensive techniques.With its high affinity and specificity, the Mouse Anti-DDDDK Tag Monoclonal Antibody is a valuable resource for researchers studying protein expression, localization, and interactions. Its versatility and reliability make it an essential tool for molecular biology and biotechnology studies.
Product Name:
Anti-FLAG (DDDDK) Magnetic Beads
SKU:
CABE037
Size:
100uL
Isotype:
IgG1
Host Species:
Mouse
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to DDDDK tag.
Sequence:
DYKD DDDK
Tested Applications:
IP
Recommended Dilution:
IP,30ul antibody (bead slurry) for 200μg-400μg extracts of whole cells
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Purification Method:
Affinity purification
Clone Number:
AMC0515
Storage Buffer:
Store at 4℃ Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,pH7.3.
Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK-Tag (1x) with 30uL Magnetic Beads-conjugated Mouse anti DDDDK-tag mAb antibody（AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:10000 dilution.
