Maf Transcription Factor Activity Assay

SKU:

TFAB00205

Product Type:

ELISA Kit

ELISA Type:

Transcription Factor Assay

Analytes:

Maf

Reactivity:

Human

Mouse

Rat

Description

Description

Datasheet

Maf Transcription Factor Activity Assay

The MAF Transcription Factor Activity Assay kit is a comprehensive solution for the precise measurement of MAF (Musculoaponeurotic Fibrosarcoma) transcription factor activity in a variety of biological samples. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.MAF is a key transcription factor that regulates gene expression in various cellular processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of MAF activity has been implicated in numerous diseases, including cancer, autoimmune disorders, and metabolic syndrome.

As such, the MAF Transcription Factor Activity Assay kit serves as a valuable tool for studying the role of MAF in disease pathology and identifying potential therapeutic targets.With its user-friendly protocol and reliable performance, the MAF Transcription Factor Activity Assay kit is an indispensable resource for researchers seeking to unravel the complexities of gene regulation and uncover novel insights into the molecular mechanisms underlying disease. Unlock the potential of your research with the MAF Transcription Factor Activity Assay kit from AssayGenie.

UniProt Protein Function:	MAF: Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells. When overexpressed, represses anti-oxidant response element (ARE)- mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters. Homodimer or heterodimer with other bHLH-Zip transcription factors. Binds DNA as a homodimer or as a heterodimer. Heterotetramer of two MAF and two USF2. Interacts with PAX6; the interaction is direct. Interacts with MYB; interaction takes place weakly in normal T-cells and increases in T-cells following stimulation through the TCR engagement. Interacts with MYB; the ternary complex formed with MYB and the CD13 promoter is regulated in response to differentiating signals. Interacts with USF2; the interaction inhibits its DNA-binding activity on the L7 promoter. Interacts with CREBBP, EP300 and ETS1. Up-regulated with tert-butyl hydroquinone (t-BHQ). Expressed in endothelial cells. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Cell development/differentiation; DNA-binding; Tumor suppressor; Oncoprotein Chromosomal Location of Human Ortholog: 16q22-q23 Cellular Component: chromatin; nucleus Molecular Function:protein binding Biological Process: regulation of transcription from RNA polymerase II promoter Disease: Ayme-gripp Syndrome; Cataract 21, Multiple Types
NCBI Summary:	The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
UniProt Code:	O75444
NCBI GenInfo Identifier:	223590080
NCBI Gene ID:	4094
NCBI Accession:	O75444.2
UniProt Secondary Accession:	O75444,Q66I47, Q9UP93,
UniProt Related Accession:	O75444
Molecular Weight:	41,961 Da
NCBI Full Name:	Transcription factor Maf
NCBI Synonym Full Names:	MAF bZIP transcription factor
NCBI Official Symbol:	MAF
NCBI Official Synonym Symbols:	CCA4; AYGRP; c-MAF; CTRCT21
NCBI Protein Information:	transcription factor Maf
UniProt Protein Name:	Transcription factor Maf
UniProt Synonym Protein Names:	Proto-oncogene c-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog
Protein Family:	Maf-like protein
UniProt Gene Name:	MAF
UniProt Entry Name:	MAF_HUMAN