The MAEL Polyclonal Antibody (PAC038994) is a valuable tool for researchers studying the MAEL protein, a key player in meiosis and RNA binding. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MAEL protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for research in reproductive biology and RNA biology.MAEL is known for its crucial role in meiotic progression and germ cell development, making it a target of interest in studies on fertility and reproductive health.
Additionally, MAEL's involvement in RNA binding suggests its potential relevance in post-transcriptional gene regulation and RNA processing. By understanding the functions of MAEL, researchers may uncover new insights into mechanisms underlying infertility, genetic disorders, and reproductive abnormalities.Overall, the MAEL Polyclonal Antibody is a valuable tool for researchers investigating the functions of MAEL in various biological processes, offering a reliable means of studying this important protein in both normal and pathological contexts.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO38994 at dilution of 1:100.
Immunofluorescence staining of Hela cells with PACO38994 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for the localization of germ-cell factors to the meiotic nuage (By similarity).
Synonyms:
Protein maelstrom homolog, MAEL
UniProt Protein Function:
MAEL: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage. Belongs to the maelstrom family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Cancer Testis Antigen (CTA)Chromosomal Location of Human Ortholog: 1q24.1Cellular Component: XY body; autosome; perinuclear region of cytoplasm; cytoplasm; chromatin; nucleusMolecular Function: DNA bindingBiological Process: multicellular organismal development; DNA methylation during gametogenesis; cell morphogenesis; regulation of organ growth; negative regulation of transcription from RNA polymerase II promoter; male meiosis; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; synapsis; RNA-mediated gene silencing; gene expression; spermatogenesis; cell differentiation; negative regulation of apoptosis
