MAEA Antibody (PACO49750)
- SKU:
- PACO49750
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
MAEA Antibody (PACO49750)
The MAEA Polyclonal Antibody (PAC049750) is a valuable tool for researchers studying MAEA, a protein involved in cell metabolism and energy production. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the MAEA protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in biochemistry and cellular metabolism.MAEA, also known as malic enzyme, plays a crucial role in the citric acid cycle, a key metabolic pathway essential for generating energy in cells.
Dysregulation of MAEA activity has been linked to metabolic disorders, cancer, and neurodegenerative diseases, highlighting its importance in health and disease. By investigating the function and regulation of MAEA, researchers can gain valuable insights into cellular metabolism and potentially identify new therapeutic targets for a range of medical conditions.
| Antibody Name: | MAEA Antibody (PACO49750) |
| Antibody SKU: | PACO49750 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Macrophage erythroblast attacher protein (299-356AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate. All lanes: MAEA antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 41, 37, 27 kDa. Observed band size: 46 kDa.. |
 | IHC image of PACO49750 diluted at 1:600 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO49750 diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. |
| Synonyms: | Macrophage erythroblast attacher (Cell proliferation-inducing gene 5 protein) (Erythroblast macrophage protein) (Human lung cancer oncogene 10 protein) (HLC-10), MAEA, EMP |
| UniProt Protein Function: | MAEA: Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA- mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cell adhesion; Actin-binding; Cell development/differentiation Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: contractile ring; cytoplasm; cytoskeleton; integral to plasma membrane; nuclear matrix; nucleus; spindle Molecular Function:actin binding Biological Process: cell adhesion; negative regulation of myeloid cell apoptosis |
| NCBI Summary: | This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q7L5Y9 |
| NCBI GenInfo Identifier: | 74754297 |
| NCBI Gene ID: | 10296 |
| NCBI Accession: | Q7L5Y9.1 |
| UniProt Secondary Accession: | Q7L5Y9,O95285, Q5JB54, Q6ZRD6, Q9BQ11, Q9H9V6, Q9H9Z4 Q9NW84, |
| UniProt Related Accession: | Q7L5Y9 |
| Molecular Weight: | 26,802 Da |
| NCBI Full Name: | Macrophage erythroblast attacher |
| NCBI Synonym Full Names: | macrophage erythroblast attacher |
| NCBI Official Symbol: | MAEAÂ Â |
| NCBI Official Synonym Symbols: | EMP; EMLP; GID9; PIG5; HLC-10Â Â |
| NCBI Protein Information: | macrophage erythroblast attacher |
| UniProt Protein Name: | Macrophage erythroblast attacher |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; Human lung cancer oncogene 10 protein; HLC-10 |
| Protein Family: | Macrophage erythroblast attacher |
| UniProt Gene Name: | MAEAÂ Â |
| UniProt Entry Name: | MAEA_HUMAN |
| Antibodies | ELISA Kits |
| MAEA Antibody (PACO00758) | MAEA Colorimetric Cell-Based ELISA |
| MAEA Antibody (PACO10330) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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