The MAEA Antibody (PAC021882) is a cutting-edge tool designed for researchers studying the MAEA protein, which plays a critical role in cellular processes and disease development. This polyclonal antibody, produced using advanced technology, is highly specific to human samples and is validated for use in various research applications, including immunohistochemistry and flow cytometry.MAEA, also known as malic enzyme, is a key enzyme in the citric acid cycle and is essential for energy production in cells. Dysregulation of MAEA has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.
By targeting the MAEA protein, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets.The MAEA Antibody offers researchers a reliable tool for studying the expression and localization of the MAEA protein in different cell types and tissues. Its high specificity and sensitivity make it ideal for use in complex biological systems, providing valuable information for basic research and drug development efforts aimed at targeting MAEA-related diseases.
Antibody Name:
MAEA Antibody (PACO21882)
Antibody SKU:
PACO21882
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human MAEA.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Bala S., Biochem. Biophys. Res. Commun. 342:1040-1048(2006).
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO205 cells, using MAEA antibody.
MAEA: Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA- mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Membrane protein, integral; Cell adhesion; Actin-binding; Cell development/differentiation
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: contractile ring; cytoplasm; cytoskeleton; integral to plasma membrane; nuclear matrix; nucleus; spindle
Molecular Function:actin binding
Biological Process: cell adhesion; negative regulation of myeloid cell apoptosis
NCBI Summary:
This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
