The MadCAM1 Polyclonal Antibody (CAB17017) is a valuable tool for researchers studying immune regulation and inflammatory responses. Raised in rabbits, this antibody is highly reactive with human samples and is validated for use in various applications, including Western blot analysis. MadCAM1, also known as mucosal addressin cell adhesion molecule 1, is a cell surface protein that plays a crucial role in the recruitment of lymphocytes to the gut mucosa. By binding to MadCAM1, this antibody enables researchers to detect and analyze the protein in different cell types, making it an ideal choice for studies in immunology and gastrointestinal research.
The significance of MadCAM1 in immune cell trafficking and inflammatory processes makes it a promising target for investigating diseases such as inflammatory bowel disease (IBD) and other gut-related disorders. Understanding the function of MadCAM1 can provide insights into therapeutic strategies aimed at modulating immune responses in these conditions.
Product Name:
MADCAM1 Rabbit Polyclonal Antibody
SKU:
CAB17017
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MADCAM1 (NP_570116.2).
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
8174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using MADCAM1 Rabbit pAb (CAB17017) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
