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Anti-MADCAM1 Antibody (CAB3005)

SKU:
CAB3005
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-MADCAM1 Antibody CAB3005
  • Cell Biology Antibodies 8 Anti-MADCAM1 Antibody CAB3005
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Description

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Anti-MADCAM1 Antibody (CAB3005)

The MadCAM1 Polyclonal Antibody (CAB3005) is a valuable tool for researchers studying MadCAM1, a cell adhesion molecule that plays a key role in the regulation of immune responses, particularly within the gastrointestinal tract. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in applications such as Western blotting.MadCAM1 is known for its involvement in the recruitment of lymphocytes to mucosal tissues, making it essential for understanding the mechanisms of immune surveillance and inflammatory reactions in the gut.

Research on MadCAM1 can provide insights into various diseases, including inflammatory bowel diseases, celiac disease, and other gastrointestinal disorders.By targeting MadCAM1, researchers can gain a better understanding of how immune cells interact with the intestinal lining and potentially develop therapeutic strategies to modulate immune responses in conditions associated with gut inflammation. The MadCAM1 Polyclonal Antibody serves as a crucial tool for investigating these pathways and advancing our knowledge of immune regulation in gastrointestinal health.

Antibody Name:Anti-MADCAM1 Antibody
Antibody SKU:CAB3005
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human MADCAM1
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:1000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, SH-SY5Y, Jurkat, A-549, mouse spleen, rat spleen
Immunogen:A synthetic peptide of human MADCAM1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8174
Uniprot:Q13477
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:21kDa/28kDa/31kDa/40kDa
Observed MW:45kDa
Synonyms:MADCAM1, MACAM1
Background:The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
UniProt Protein Function:MADCAM1: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; integral to membrane; plasma membrane

Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; positive regulation of leukocyte migration; cell-matrix adhesion; signal transduction; receptor clustering; keratinocyte differentiation; heterotypic cell-cell adhesion; immune response; leukocyte tethering or rolling; cell adhesion; aging

NCBI Summary:The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q13477
NCBI GenInfo Identifier:218511712
NCBI Gene ID:8174
NCBI Accession:Q13477.2
UniProt Secondary Accession:Q13477,O60222, O75867, Q5UGI7, A5PKV4, B2RPL9,
UniProt Related Accession:Q13477
Molecular Weight:382
NCBI Full Name:Mucosal addressin cell adhesion molecule 1
NCBI Synonym Full Names:mucosal vascular addressin cell adhesion molecule 1
NCBI Official Symbol:MADCAM1  
NCBI Official Synonym Symbols:MACAM1  
NCBI Protein Information:mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
UniProt Protein Name:Mucosal addressin cell adhesion molecule 1
Protein Family:Mucosal addressin cell adhesion molecule
UniProt Gene Name:MADCAM1  
UniProt Entry Name:MADCA_HUMAN
Anti-MADCAM1 Antibody (CAB3005)Western blot analysis of extracts of various cell lines, using MADCAM1 antibody (CAB3005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-MADCAM1 Antibody (CAB3005)Immunohistochemistry of paraffin-embedded human appendix using MADCAM1 antibody (CAB3005) at dilution of 1:100 (40x lens).

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