The MACF1 Polyclonal Antibody (PAC062835) is a valuable tool for researchers studying MACF1, a key protein involved in various cellular processes such as cell migration, cell signaling, and cytoskeleton organization. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot and immunofluorescence applications.MACF1, also known as microtubule-actin crosslinking factor 1, is a large multidomain cytoskeletal linker protein that plays a crucial role in maintaining cell shape and movement. Its involvement in intracellular transport and signaling pathways makes it a significant player in cellular physiology and pathology.
By targeting MACF1 with this antibody, researchers can better understand its function and contribution to diseases like cancer, neurodegenerative disorders, and developmental abnormalities.Overall, the MACF1 Polyclonal Antibody is a reliable tool for investigating the role of MACF1 in cellular processes and disease mechanisms, ultimately leading to potential therapeutic strategies targeting this protein for various conditions.
Antibody Name:
MACF1 Antibody (PACO62835)
Antibody SKU:
PACO62835
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 protein (1936-2150AA)
IHC image of PACO62835 diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Isoform 2 is a F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate MT dynamics and polarize hair follicle stem cells (By similarity).
This gene encodes a large protein containing numerous spectrin and leucine-rich repeat (LRR) domains. The encoded protein is a member of a family of proteins that form bridges between different cytoskeletal elements. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. Alternative splicing results in multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]
