M1-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18200)
The M1 Linkage-Specific Polyubiquitin Polyclonal Antibody (CAB18200) is a valuable tool for researchers studying the role of polyubiquitination in cellular processes. This antibody, raised in rabbits, is highly specific for M1-linked polyubiquitin chains and is validated for use in various applications, including Western blotting.Polyubiquitination, the process by which proteins are tagged with ubiquitin chains, plays a crucial role in protein degradation, signal transduction, and DNA repair. M1-linked polyubiquitin chains, in particular, are involved in immune signaling pathways and inflammatory responses, making them a key focus in immunology and cancer research.
By targeting M1-linked polyubiquitin chains, researchers can investigate the mechanisms by which these chains regulate immune responses and cellular signaling. The M1 Linkage-Specific Polyubiquitin Polyclonal Antibody allows for the detection and analysis of M1-linked polyubiquitin chains in a variety of experimental settings, providing valuable insights into the role of polyubiquitination in health and disease.
Product Name:
M1-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody
SKU:
CAB18200
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human M1-linkage Specific Polyubiquitin (NP_066289.3/NP_061828.1).
Ubiquitination,onetypeofthemostcommonpost-translationalmodification,mediatestheregulationofproteinhomeostasisinvivo. Substrate proteins can be modified with single ubiquitin moieties or with polymeric ubiquitin chains. Within polyubiquitin chains, ubiquitin can form eight different linkage types, using one of seven internal lysine residues (K6, K11, K27, K29, K33, K48, K63) or methionine at position 1 (M1).Here we focus on a distinct type of ubiquitination that is characterized by an inter-ubiquitin linkage through the N-terminal methionine, called M1-linked or linear ubiquitination. Formation, recognition, and disassembly of linear ubiquitin chains are highly specific processes that are implicated in immune signaling, cell death regulation and protein quality control. Consistent with their role in influencing signaling events, linear ubiquitin chains are formed in a transient and spatially regulated manner, making their detection and quantification challenging.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using M1-linkage Specific Polyubiquitin Rabbit pAb (CAB18200) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
