M1-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18200)
- SKU:
- CAB18200
- Product Type:
- Antibody
- Reactivity:
- Universal
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Product Name: | M1-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody |
SKU: | CAB18200 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human M1-linkage Specific Polyubiquitin (NP_066289.3/NP_061828.1). |
Sequence: | MQIF VKTL TGKT ITLE VEPS DTIE NVKA KIQD KEGI PPDQ QRLI FAGK QLED GRTL SDYN IQKE STLH LVLR LRGG MQIF VKTL TGKT ITLE VEPS DTIE |
Tested Applications: | WB ELISA DB |
Recommended Dilution: | WB,1:500 - 1:2000 DB,1:500 - 1:1000 |
Positive Sample: | HeLa,NIH/3T3,RAW264.7,C6,Mouse brain,Mouse kidney,Rat brain,Rat kidney |
Observed MW: | Refer to figures |
Ubiquitination,onetypeofthemostcommonpost-translationalmodification,mediatestheregulationofproteinhomeostasisinvivo. Substrate proteins can be modified with single ubiquitin moieties or with polymeric ubiquitin chains. Within polyubiquitin chains, ubiquitin can form eight different linkage types, using one of seven internal lysine residues (K6, K11, K27, K29, K33, K48, K63) or methionine at position 1 (M1).Here we focus on a distinct type of ubiquitination that is characterized by an inter-ubiquitin linkage through the N-terminal methionine, called M1-linked or linear ubiquitination. Formation, recognition, and disassembly of linear ubiquitin chains are highly specific processes that are implicated in immune signaling, cell death regulation and protein quality control. Consistent with their role in influencing signaling events, linear ubiquitin chains are formed in a transient and spatially regulated manner, making their detection and quantification challenging.
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |