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Lyn Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB00738
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Immunology
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€599
Frequently bought together:

Description

system_update_altDatasheet

Lyn Colorimetric Cell-Based ELISA Kit

The Lyn Colorimetric Cell-Based ELISA Kit is a cutting-edge tool for researchers looking to accurately measure Lyn protein levels in cell lysates. Lyn is a member of the Src family of protein kinases and plays a critical role in cell signaling pathways, making it a key target for studying various cellular processes.This kit offers high sensitivity and specificity, allowing for precise and reliable detection of Lyn levels in a variety of cell types. With its user-friendly protocol and quick assay time, researchers can obtain accurate results with ease, making it ideal for a wide range of cell-based research applications.

By using the Lyn Colorimetric Cell-Based ELISA Kit, researchers can gain valuable insights into Lyn-mediated signaling pathways and their impact on cellular functions. This kit is a valuable tool for studying cancer, immune response, and other diseases where Lyn is known to play a crucial role.

Product Name:Lyn Colorimetric Cell-Based ELISA
Product Code:CBCAB00738
ELISA Type:Cell-Based
Target:Lyn
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The Lyn Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Lyn protein expression profile in cells. The kit can be used for measuring the relative amounts of Lyn in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on Lyn.

Qualitative determination of Lyn concentration is achieved by an indirect ELISA format. In essence, Lyn is captured by Lyn-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 4067, UniProt ID: P07948, OMIM: 165120, Unigene: Hs.491767
Gene Symbol:LYN
Sub Type:None
UniProt Protein Function:LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.
UniProt Protein Details:

Protein type:Protein kinase, tyrosine (non-receptor); Kinase, protein; Oncoprotein; Protein kinase, TK; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; Golgi apparatus; lipid raft; mast cell granule; mitochondrial crista; mitochondrial intermembrane space; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density

Molecular Function:ATP binding; enzyme binding; gamma-tubulin binding; glycosphingolipid binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; platelet-derived growth factor receptor binding; protein binding; protein-tyrosine kinase activity; receptor binding; receptor signaling protein tyrosine kinase activity; SH3 domain binding; ubiquitin protein ligase binding

Biological Process: adaptive immune response; axon guidance; B cell homeostasis; B cell receptor signaling pathway; blood coagulation; cellular response to extracellular stimulus; cytokine secretion; ephrin receptor signaling pathway; erythrocyte differentiation; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; histamine secretion by mast cell; immune response-regulating cell surface receptor signaling pathway; innate immune response; leukocyte migration; lipopolysaccharide-mediated signaling pathway; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of immune response; negative regulation of MAP kinase activity; negative regulation of myeloid leukocyte differentiation; negative regulation of protein amino acid phosphorylation; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; neurite development; oligodendrocyte development; peptidyl-tyrosine phosphorylation; platelet activation; platelet degranulation; positive regulation of B cell receptor signaling pathway; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tyrosine phosphorylation of STAT protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of B cell apoptosis; regulation of B cell receptor signaling pathway; regulation of cell adhesion mediated by integrin; regulation of cytokine production; regulation of cytokine secretion; regulation of erythrocyte differentiation; regulation of inflammatory response; regulation of mast cell activation; regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; regulation of release of sequestered calcium ion into cytosol; response to amino acid stimulus; response to axon injury; response to carbohydrate stimulus; response to DNA damage stimulus; response to drug; response to hormone stimulus; response to insulin stimulus; response to organic cyclic substance; response to sterol depletion; response to toxin; signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell costimulation; tolerance induction to self antigen; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction

NCBI Summary:This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
UniProt Code:P07948
NCBI GenInfo Identifier:125480
NCBI Gene ID:4067
NCBI Accession:P07948.3
UniProt Secondary Accession:P07948,A0AVQ5,
UniProt Related Accession:P07948
Molecular Weight:56,033 Da
NCBI Full Name:Tyrosine-protein kinase Lyn
NCBI Synonym Full Names:LYN proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol:LYN  
NCBI Official Synonym Symbols:JTK8; p53Lyn; p56Lyn  
NCBI Protein Information:tyrosine-protein kinase Lyn
UniProt Protein Name:Tyrosine-protein kinase Lyn
UniProt Synonym Protein Names:Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn
Protein Family:Tyrosine-protein kinase
UniProt Gene Name:LYN  
UniProt Entry Name:LYN_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-Lyn Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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