The LTN1 Polyclonal Antibody (PACO59940) is a valuable tool for researchers studying LTN1, a protein involved in protein degradation and quality control mechanisms in cells. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the LTN1 protein, this antibody enables the detection and analysis of LTN1 in various cell types, making it an essential asset for research in cell biology and protein degradation pathways.
LTN1, also known as E3 ubiquitin-protein ligase Listerin, plays a crucial role in maintaining cellular homeostasis by targeting misfolded or damaged proteins for degradation. Dysregulation of LTN1 activity has been implicated in various diseases, making it a promising therapeutic target for conditions such as neurodegenerative disorders and cancer. Understanding the functions of LTN1 is vital for unraveling its role in cellular quality control mechanisms and developing targeted therapies for related diseases.
Antibody Name:
LTN1 Antibody (PACO59940)
Antibody SKU:
PACO59940
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human E3 ubiquitin-protein ligase listerin protein (462-591AA)
IHC image of PACO59940 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO59940 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product (By similarity).
ZNF294: a zinc-finger protein which contains one RING/zf-C3HC4 domain. The RING finger is a cysteine-rich domain of 40 to 60 residues that coordinates two zinc ions, and has the consensus sequence: C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-48)-C-X2-C. Many RING finger proteins play a key role in the ubiquitination pathway. A candidate colon cancer tumor suppressor genes identified using nonsense-mediated mRNA decay in colon cancer cells.Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 21q22.11Molecular Function: protein binding
UniProt Protein Details:
NCBI Summary:
Like most RING finger proteins, LTN1 functions as an E3 ubiquitin ligase (Chu et al., 2009 [PubMed 19196968]).[supplied by OMIM, Nov 2010]
