Anti-LSM4 Antibody (CAB13588)
- SKU:
- CAB13588
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-LSM4 Antibody (CAB13588)
The LSM4 Polyclonal Antibody (CAB13588) is a valuable tool for researchers investigating LSM4, a key protein involved in mRNA splicing and processing. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the LSM4 protein, this antibody enables precise detection and analysis in various cell types, making it an essential tool for studies in molecular biology and RNA processing research.LSM4 is a critical component of the mRNA processing machinery, playing a vital role in regulating gene expression and RNA stability.
Dysregulation of LSM4 function has been implicated in a variety of diseases, including cancer and developmental disorders. By studying the role of LSM4 in mRNA splicing and processing, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets for precision medicine approaches.Overall, the LSM4 Polyclonal Antibody (CAB13588) holds great potential for advancing our understanding of RNA processing and its impact on human health, making it a valuable asset for researchers in the fields of molecular biology and genomics.
| Antibody Name: | Anti-LSM4 Antibody |
| Antibody SKU: | CAB13588 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| Reactivity: | Human |
| Positive Samples: | HepG2, MCF-7, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLPL SLLK TAQN HPML VELK NGET YNGH LVSC DNWM NINL REVI CTSR DGDK FWRM PECY IRGS TIKY LRIP DEII DMVK EEVV AKGR GRGG LQQQ KQQK GRGM GGAG RGVF GGRG RGGI PGTG RGQP EKKP GRQA GKQ |
| Gene ID: | 25804 |
| Uniprot: | Q9Y4Z0 |
| Cellular Location: | Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 14kDa |
| Synonyms: | LSM4, GRP, YER112W |
| Background: | This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | LSM4: Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family. |
| UniProt Protein Details: | Protein type:RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: small nuclear ribonucleoprotein complex; spliceosome; cytosol Molecular Function:protein binding Biological Process: RNA splicing; gene expression; mRNA processing; mRNA catabolic process, deadenylation-dependent decay |
| NCBI Summary: | This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q9Y4Z0 |
| NCBI GenInfo Identifier: | 10720082 |
| NCBI Gene ID: | 25804 |
| NCBI Accession: | Q9Y4Z0.1 |
| UniProt Related Accession: | Q9Y4Z0 |
| Molecular Weight: | Calculated MW: 15kDaObserved MW: 15kDa |
| NCBI Full Name: | U6 snRNA-associated Sm-like protein LSm4 |
| NCBI Synonym Full Names: | LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) |
| NCBI Official Symbol: | LSM4Â Â |
| NCBI Official Synonym Symbols: | GRP; YER112WÂ Â |
| NCBI Protein Information: | U6 snRNA-associated Sm-like protein LSm4; glycine-rich protein |
| UniProt Protein Name: | U6 snRNA-associated Sm-like protein LSm4 |
| UniProt Synonym Protein Names: | Glycine-rich protein; GRP |
| Protein Family: | Sm-like protein |
| UniProt Gene Name: | LSM4Â Â |
| UniProt Entry Name: | LSM4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LSM4 antibody (CAB13588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human colon using LSM4 Rabbit pAb (CAB13588) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using LSM4 antibody (CAB13588) at dilution of 1:50. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug LSM4 antibody (CAB13588). Western blot was performed from the immunoprecipitate using LSM4 antibody (CAB13588) at a dilition of 1:1000. |
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