Anti-LRSAM1 Antibody (CAB9885)

Antibody Name:	Anti-LRSAM1 Antibody
Antibody SKU:	CAB9885
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human LRSAM1 (NP_612370.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HT-1080, Mouse brain, Rat spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human LRSAM1 (NP_612370.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPLF FRKR KPSE EARK RLEY QMCL AKEA GADD ILDI SKCE LSEI PFGA FATC KVLQ KKVL IVHT NHLT SLLP KSCS LLSL ATIK VLDL HDNQ LTAL PDDL GQLT ALQV LNVE RNQL MQLP RSIG NLTQ LQTL NVKD NKLK ELPD TVGE LRSL RTLN ISGN EIQR LPQM LAHV RTLE MLSL DASA MVYP PREV CGAG TAAI LQFL CKES GLEY YPPS QYLL PILE QDGI ENSR DSPD GPTD RFSR EELE WQNR FSDY EKRK EQKM LEKL EFER RLEL GQRE HTQL LQQS SS
Gene ID:	90678
Uniprot:	Q6UWE0
Cellular Location:	Cytoplasm
Calculated MW:	35kDa/80kDa/83kDa
Observed MW:	84kDa

Synonyms:

LRSAM1, CMT2P, RIFLE, TAL

Background:

This gene encodes a ring finger protein involved in a variety of functions, including regulation of signaling pathways and cell adhesion, mediation of self-ubiquitylation, and involvement in cargo sorting during receptor endocytosis. Mutations in this gene have been associated with Charcot-Marie-Tooth disease. Multiple transcript variants encoding different isoforms have been identified for this gene.

UniProt Protein Function:	LRSAM1: E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. Defects in LRSAM1 are a cause of Charcot-Marie-Tooth disease type 2P (CMT2P). CMT2P is an axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ligase; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase Chromosomal Location of Human Ortholog: 9q33.3 Cellular Component: cytoplasm; membrane Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: negative regulation of endocytosis; non-lytic virus budding; protein autoubiquitination; protein catabolic process; protein polyubiquitination Disease: Charcot-marie-tooth Disease, Axonal, Type 2p
NCBI Summary:	This gene encodes a ring finger protein involved in a variety of functions, including regulation of signaling pathways and cell adhesion, mediation of self-ubiquitylation, and involvement in cargo sorting during receptor endocytosis. Mutations in this gene have been associated with Charcot-Marie-Tooth disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:	Q6UWE0
NCBI GenInfo Identifier:	62511890
NCBI Gene ID:	90678
NCBI Accession:	Q6UWE0.1
UniProt Secondary Accession:	Q6UWE0,Q5VVV0, Q8NB40, Q96GT5, Q96MX5, Q96MZ7,
UniProt Related Accession:	Q6UWE0
Molecular Weight:	35,069 Da
NCBI Full Name:	E3 ubiquitin-protein ligase LRSAM1
NCBI Synonym Full Names:	leucine rich repeat and sterile alpha motif containing 1
NCBI Official Symbol:	LRSAM1
NCBI Official Synonym Symbols:	TAL; CMT2P; RIFLE
NCBI Protein Information:	E3 ubiquitin-protein ligase LRSAM1
UniProt Protein Name:	E3 ubiquitin-protein ligase LRSAM1
UniProt Synonym Protein Names:	Leucine-rich repeat and sterile alpha motif-containing protein 1
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	LRSAM1
UniProt Entry Name:	LRSM1_HUMAN

Anti-LRSAM1 Polyclonal Antibody (CAB9885)

Western blot analysis of extracts of various cell lines, using LRSAM1 antibody (CAB9885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)