The LRRC45 Polyclonal Antibody (PACO58396) is a valuable tool for researchers investigating LRRC45, a protein that plays a crucial role in cellular functions. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been rigorously validated for use in Western blot applications. By binding specifically to LRRC45, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and cancer research.LRRC45, also known as leucine-rich repeat-containing protein 45, is involved in various cellular processes, including signal transduction and protein-protein interactions. Its role in these mechanisms makes it an important target for research aimed at understanding disease mechanisms and identifying potential therapeutic targets.
By elucidating the function of LRRC45, researchers can gain insights into pathways that may be dysregulated in conditions such as cancer, neurological disorders, and autoimmune diseases.Investigating LRRC45 with the PACO58396 Polyclonal Antibody can provide valuable insights into the protein's function and its potential implications for disease pathology. With its high reactivity and specificity, this antibody is a valuable asset for researchers seeking to uncover the intricacies of LRRC45 biology and its relevance to human health.
IHC image of PACO58396 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO58396 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO58396 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Component of the proteinaceous fiber-like linker between two centrioles, required for centrosome cohesion.
Synonyms:
Leucine-rich repeat-containing protein 45, LRRC45
UniProt Protein Function:
ARF3: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.Protein type: G protein; G protein, monomeric; G protein, monomeric, ARF; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 12q13Cellular Component: Golgi membrane; perinuclear region of cytoplasmMolecular Function: GTPase activity; GTP bindingBiological Process: vesicle-mediated transport; protein transport; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process
