The LRAT Polyclonal Antibody (CAB6647) is a valuable tool for researchers studying the lecithin retinol acyltransferase (LRAT) protein. LRAT is an enzyme that plays a crucial role in the visual cycle by catalyzing the esterification of retinol (vitamin A) to form the storage form, retinyl esters. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the LRAT protein, researchers can easily detect and analyze its expression in various cell types. This makes the LRAT Polyclonal Antibody an excellent choice for studies in vision research, retinoid metabolism, and metabolic disorders related to vitamin A deficiency.
Understanding the function of LRAT is essential for unraveling its involvement in diseases such as night blindness, retinitis pigmentosa, and age-related macular degeneration. By targeting LRAT, researchers may uncover potential therapeutic strategies for treating these vision-related conditions. The LRAT Polyclonal Antibody offers a reliable tool for elucidating the role of LRAT in these disease pathways and advancing our knowledge in the field of retinoid biology.
Product Name:
LRAT Rabbit Polyclonal Antibody
SKU:
CAB6647
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human LRAT (NP_004735.2).
The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9227
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of rat testis, using Lrat antibody (CAB6647) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
