The LRAT Polyclonal Antibody (CAB13175) is a valuable tool for researchers studying lecithin retinol acyltransferase (LRAT), an enzyme involved in the visual cycle and vitamin A metabolism. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.LRAT plays a crucial role in the conversion of dietary vitamin A into retinyl esters for storage in the liver and retinol delivery to the eye for visual pigment regeneration.
Dysregulation of LRAT has been implicated in conditions such as night blindness and retinitis pigmentosa, highlighting its importance in maintaining vision health.By detecting and quantifying LRAT protein levels in various cell types and tissues, researchers can explore the enzyme's function in vision maintenance and vitamin A metabolism. This antibody is an essential tool for investigations into retinoid biology, eye diseases, and metabolic disorders related to vitamin A deficiency.
Product Name:
LRAT Rabbit Polyclonal Antibody
SKU:
CAB13175
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 31-150 of human LRAT (NP_004735.2).
The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9227
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LRAT Rabbit pAb (CAB13175) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
