The LPCAT1 Polyclonal Antibody (CAB4987) is specifically designed for research involving LPCAT1, a key enzyme involved in the biosynthesis of phosphatidylcholine, a major component of cell membranes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blot and immunofluorescence.LPCAT1 is essential for maintaining cellular membrane integrity and regulating lipid composition, making it a crucial player in processes such as cell proliferation, apoptosis, and lipid metabolism. Dysregulation of LPCAT1 has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.
By targeting LPCAT1 and studying its functions, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially develop novel therapeutic strategies.The LPCAT1 Polyclonal Antibody offers researchers a reliable tool for studying the expression and localization of LPCAT1 in different cell types and tissues. Its ability to specifically detect LPCAT1 protein makes it an invaluable asset for investigating the role of LPCAT1 in cellular physiology and disease pathogenesis. Whether studying lipid metabolism, cell signaling, or disease mechanisms, this antibody is a valuable resource for advancing research in biochemistry, cell biology, and lipid biology.
Product Name:
LPCAT1 Rabbit Polyclonal Antibody
SKU:
CAB4987
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 325-534 of human LPCAT1 (NP_079106.3).
Endoplasmic reticulum membrane, Golgi apparatus membrane, Lipid droplet, Single-pass type II membrane protein.
Calculated MW:
59kDa
Observed MW:
59kDa
This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers.
Purification Method:
Affinity purification
Gene ID:
79888
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using LPCAT1 antibody (CAB4987) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
