The LPAR5 Polyclonal Antibody (PAC061947) is a valuable tool for researchers studying Lysophosphatidic Acid Receptor 5 (LPAR5), a G protein-coupled receptor involved in various cellular processes, including cell proliferation, survival, migration, and immune responses. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications.LPAR5 is known for its role in mediating signaling pathways that promote cancer progression, making it a potential target for cancer therapy research. By binding specifically to the LPAR5 protein, this antibody enables researchers to detect and analyze its expression in different cell types, providing important insights into its function in cancer biology.
Additionally, LPAR5 has been implicated in inflammatory responses and vascular development, further highlighting its significance in various physiological and pathological processes.With its ability to facilitate the study of LPAR5 in different contexts, this polyclonal antibody is essential for advancing our understanding of the molecular mechanisms involving this receptor and its potential implications for therapeutic interventions in cancer and other diseases.
IHC image of PACO61947 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO61947 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Receptor for lysophosphatidic acid, (LPA), a mediator of diverse cellular activities.
GPR92: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Belongs to the G-protein coupled receptor 1 family.Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1Chromosomal Location of Human Ortholog: 12p13.31Cellular Component: integral to membrane; plasma membraneMolecular Function: G-protein coupled receptor activityBiological Process: G-protein coupled receptor protein signaling pathway
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the rhodopsin class of G protein-coupled transmembrane receptors. This protein transmits extracellular signals from lysophosphatidic acid to cells through heterotrimeric G proteins and mediates numerous cellular processes. Many G protein receptors serve as targets for pharmaceutical drugs. Transcript variants of this gene have been described.[provided by RefSeq, Dec 2008]
