The LOXL1 Polyclonal Antibody (CAB10191) is specially crafted for research pertaining to LOXL1, a key enzyme involved in extracellular matrix remodeling and connective tissue function. This antibody is produced in rabbits and demonstrates high specificity towards human samples, making it an excellent tool for use in Western blot applications. By binding to the LOXL1 protein, researchers can accurately detect and study its expression in various cell types, making it an invaluable asset for investigations in the fields of molecular biology, tissue engineering, and fibrosis research.LOXL1, also known as lysyl oxidase-like 1, plays a crucial role in maintaining tissue integrity and regulating collagen cross-linking, essential processes for overall tissue health and function.
Dysregulation of LOXL1 has been implicated in various pathological conditions, including fibrotic disorders, cardiovascular diseases, and certain types of cancers. Understanding the function and regulation of LOXL1 is key to developing targeted therapies for these diseases and advancing our knowledge of tissue remodeling mechanisms.In summary, the LOXL1 Polyclonal Antibody (CAB10191) offers a reliable tool for researchers seeking to explore the functions and implications of LOXL1 in health and disease. Its high specificity and sensitivity make it an ideal choice for studies aiming to unravel the complexities of extracellular matrix dynamics and tissue homeostasis.
Product Name:
LOXL1 Rabbit Polyclonal Antibody
SKU:
CAB10191
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 95-370 of human LOXL1 (NP_005567.2).
This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome.
Purification Method:
Affinity purification
Gene ID:
4016
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using LOXL1 Rabbit pAb (CAB10191)at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): U-937Exposure time: 30s.
