The LMO7 Polyclonal Antibody (PACO60236) is a valuable tool for researchers studying the LMO7 protein, which plays a crucial role in cell adhesion and signaling pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot and immunofluorescence applications. By specifically binding to the LMO7 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential reagent for studies in cell biology and cancer research.
LMO7 is known to be involved in cell adhesion and cytoskeletal organization, making it a key player in cellular processes such as migration and differentiation. Dysregulation of LMO7 expression has been implicated in various diseases, including cancer and developmental disorders. By studying the function and regulation of LMO7, researchers can gain valuable insights into the mechanisms underlying these pathologies and potentially identify novel therapeutic targets for intervention.
IHC image of PACO60236 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO60236 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO60236 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
cytoplasm, cytosol, focal adhesion, nucleus, ubiquitin ligase complex, ubiquitin-protein transferase activity, post-translational protein modification, protein polyubiquitination, protein ubiquitination
Synonyms:
LIM domain only protein 7 (LMO-7) (F-box only protein 20) (LOMP), LMO7, FBX20 FBXO20 KIAA0858
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein containing a calponin homology (CH) domain, a PDZ domain, and a LIM domain, and may be involved in protein-protein interactions. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, however, the full-length nature of some variants is not known. [provided by RefSeq, Jan 2009]
