LMCD1 Antibody (PACO58520)
- SKU:
- PACO58520
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | LMCD1 Antibody (PACO58520) |
| Antibody SKU: | PACO58520 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human LIM and cysteine-rich domains protein 1 protein (105-268AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58520 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO58520 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO58520 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway. |
| Synonyms: | LIM and cysteine-rich domains protein 1 (Dyxin), LMCD1 |
| UniProt Protein Function: | LMCD1: Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue- specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.Protein type: Unknown functionChromosomal Location of Human Ortholog: 3p26-p24Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleusMolecular Function: zinc ion binding; transcription corepressor activityBiological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the LIM-domain family of zinc finger proteins. The encoded protein contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. The protein may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | Q9NZU5 |
| NCBI GenInfo Identifier: | 20978521 |
| NCBI Gene ID: | 29995 |
| NCBI Accession: | Q9NZU5.1 |
| UniProt Secondary Accession: | Q9NZU5,B4DG80 |
| UniProt Related Accession: | Q9NZU5 |
| Molecular Weight: | 32,882 Da |
| NCBI Full Name: | LIM and cysteine-rich domains protein 1 |
| NCBI Synonym Full Names: | LIM and cysteine-rich domains 1 |
| NCBI Official Symbol: | LMCD1Â Â |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | LIM and cysteine-rich domains protein 1; dyxin |
| UniProt Protein Name: | LIM and cysteine-rich domains protein 1 |
| UniProt Synonym Protein Names: | Dyxin |
| Protein Family: | LIM and cysteine-rich domains protein |
| UniProt Gene Name: | LMCD1Â Â |
| UniProt Entry Name: | LMCD1_HUMAN |