The LMCD1 Polyclonal Antibody (PACO58520) is a valuable tool for researchers studying LMCD1, a key protein involved in cardiac hypertrophy regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the LMCD1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it well-suited for investigations in cardiovascular biology and disease research.LMCD1, also known as LIM and cysteine-rich domains protein 1, plays a crucial role in modulating cardiac muscle growth and function. Its involvement in cardiac hypertrophy processes makes it a compelling target for studying heart disease, heart failure, and other cardiovascular conditions.
By elucidating the functions of LMCD1, researchers can gain insights into potential therapeutic strategies aimed at managing cardiac hypertrophy and related disorders.Overall, the LMCD1 Polyclonal Antibody (PACO58520) offers a valuable tool for researchers seeking to understand the molecular mechanisms underlying cardiac hypertrophy and related cardiovascular diseases. Its specificity and sensitivity make it an excellent choice for investigative studies in the field of cardiovascular research.
IHC image of PACO58520 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO58520 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO58520 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.
Synonyms:
LIM and cysteine-rich domains protein 1 (Dyxin), LMCD1
UniProt Protein Function:
LMCD1: Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue- specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.Protein type: Unknown functionChromosomal Location of Human Ortholog: 3p26-p24Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleusMolecular Function: zinc ion binding; transcription corepressor activityBiological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the LIM-domain family of zinc finger proteins. The encoded protein contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. The protein may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
