The LIPF Polyclonal Antibody (CAB7016) is a valuable tool for researchers studying Lipase F, an enzyme involved in lipid metabolism. This antibody is raised in rabbits and is highly specific for Lipase F in human samples, making it suitable for a variety of research applications, including Western blot analysis. By binding to Lipase F, this antibody enables accurate detection and analysis of the enzyme in different cell types, which is essential for research in areas such as lipid metabolism, obesity, and cardiovascular disease.
Lipase F is an important enzyme that plays a key role in the breakdown of lipids and fatty acids, making it a promising target for investigations into metabolic disorders and related conditions. Research on Lipase F can provide valuable insights into how lipid metabolism is regulated and how dysregulation of this process can contribute to various health issues. The LIPF Polyclonal Antibody is a reliable tool for researchers aiming to deepen their understanding of Lipase F and its implications for human health.
Product Name:
LIPF Rabbit Polyclonal Antibody
SKU:
CAB7016
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 219-398 of human LIPF (NP_004181.1).
MCF7,Mouse small intestine,Mouse lung,Mouse heart,Mouse liver,Mouse stomach,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8513
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using LIPF antibody (CAB7016) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
