The LIPA Polyclonal Antibody (CAB6385) is a powerful tool for researchers studying LIPA, a key enzyme involved in lipid metabolism and cellular homeostasis. This antibody, produced in rabbits, is highly specific for detecting LIPA in human samples and is validated for use in Western blot applications. By targeting the LIPA protein, researchers can gain insights into lipid processing, storage, and utilization within cells, with potential implications in metabolic disorders and lipid-related diseases.
LIPA, also known as lysosomal acid lipase, plays a crucial role in breaking down lipids within lysosomes, organelles that are responsible for cellular waste disposal. Dysregulation of LIPA activity can lead to lipid accumulation and disruptions in cellular function, contributing to conditions such as fatty liver disease and atherosclerosis. By studying LIPA expression and activity, researchers can uncover new therapeutic targets and strategies for treating lipid metabolism disorders.
Product Name:
LIPA Rabbit Polyclonal Antibody
SKU:
CAB6385
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-399 of human LIPA (NP_001121077.1).
This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3988
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using LIPA antibody (CAB6385) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
