Anti-LIN28B Antibody (CAB12958)

Antibody Name:	Anti-LIN28B Antibody
Antibody SKU:	CAB12958
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-250 of human LIN28B (NP_001004317.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-250 of human LIN28B (NP_001004317.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VANC PHKN VAQP PASS QGRQ EAES QPCT STLP REVG GGHG CTSP PFPQ EARA EISE RSGR SPQE ASST KSSI APEE QSKK GPSV QKRK KT
Gene ID:	389421
Uniprot:	Q6ZN17
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	19kDa/27kDa
Observed MW:	Refer to Figures

Synonyms:

LIN28B, CSDD2

Background:

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation.

UniProt Protein Function:	LIN28B: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 6q21 Molecular Function:protein binding; RNA binding Biological Process: pre-microRNA processing; RNA 3'-end processing
NCBI Summary:	The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]
UniProt Code:	Q6ZN17
NCBI GenInfo Identifier:	74758701
NCBI Gene ID:	389421
NCBI Accession:	Q6ZN17.1
UniProt Secondary Accession:	Q6ZN17,Q5TCM4, A1L165, B2RPN6,
UniProt Related Accession:	Q6ZN17
Molecular Weight:	19,511 Da
NCBI Full Name:	Protein lin-28 homolog B
NCBI Synonym Full Names:	lin-28 homolog B
NCBI Official Symbol:	LIN28B
NCBI Official Synonym Symbols:	CSDD2
NCBI Protein Information:	protein lin-28 homolog B
UniProt Protein Name:	Protein lin-28 homolog B
Protein Family:	Protein
UniProt Gene Name:	LIN28B
UniProt Entry Name:	LN28B_HUMAN

Antibodies

Anti-LIN28B Antibody (CAB12957)

Anti-Lin28B Antibody (CAB9524)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)