The LIMK1 Polyclonal Antibody (CAB16664) is a valuable tool for researchers studying LIMK1, a protein involved in the regulation of actin dynamics and cell motility. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.LIMK1 is known to play a key role in processes such as cell migration, invasion, and cytoskeletal organization, making it a key target for studies in cell biology and cancer research.
By binding specifically to the LIMK1 protein, this antibody enables accurate detection and analysis in a variety of cell types.Understanding the function of LIMK1 is crucial for unraveling its role in various physiological and pathological processes. Research involving this protein may provide insights into diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, ultimately paving the way for the development of targeted therapies.
Product Name:
LIMK1 Rabbit Polyclonal Antibody
SKU:
CAB16664
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 510-647 of LIMK1 (NP_002305.1).
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
3984
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using LIMK1 Rabbit pAb (CAB16664) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
