The Lig3 Polyclonal Antibody (CAB13507) is an essential tool for researchers studying DNA ligase 3 (Lig3), an enzyme involved in the DNA repair process. This antibody, generated in rabbit hosts, shows high reactivity with human samples and is validated for use in techniques such as Western blotting. By specifically binding to Lig3 protein, this antibody enables accurate detection and analysis in various cell types, making it an invaluable asset for research in fields like genetics, molecular biology, and cancer studies.
Lig3 plays a crucial role in repairing DNA damage, making it a key player in maintaining genomic stability and preventing mutations that can lead to diseases like cancer. Understanding the function and regulation of Lig3 is essential for developing targeted therapies and treatments for genetic disorders and cancer. The use of the Lig3 Polyclonal Antibody allows researchers to delve deeper into the mechanisms of DNA repair and explore new avenues for therapeutic interventions in the future.
Product Name:
LIG3 Rabbit Polyclonal Antibody
SKU:
CAB13507
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 254-460 of human DNA Ligase III/LIG3 (NP_039269.2).
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
3980
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CAB13507) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
