The DNA Ligase 1 Polyclonal Antibody (CAB1858) is a key tool for researchers studying DNA repair mechanisms and processes. This antibody, raised in rabbits, is specifically designed to target DNA Ligase 1, an essential enzyme involved in the joining of DNA strands during DNA replication and repair.With high reactivity towards human samples, this antibody is validated for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Its ability to specifically bind to DNA Ligase 1 enables researchers to accurately detect and analyze this crucial enzyme in a variety of cell types.DNA Ligase 1 is essential for maintaining genomic integrity and stability, making it a vital component in understanding DNA repair processes and mechanisms.
Dysregulation of DNA Ligase 1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and developmental abnormalities.By using the DNA Ligase 1 Polyclonal Antibody, researchers can gain valuable insights into the role of DNA Ligase 1 in DNA repair pathways and its potential as a therapeutic target for diseases associated with DNA damage and genomic instability. This antibody is a valuable tool for studies in molecular biology, genetics, and cancer research, providing crucial information for the development of novel treatment strategies.
Product Name:
LIG1 Rabbit Polyclonal Antibody
SKU:
CAB1858
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_000225.1).
This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using LIG1 Rabbit pAb (CAB1858) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
