The LGR5 Polyclonal Antibody (CAB10545) is a valuable tool for researchers studying LGR5, a stem cell marker associated with various tissues including the intestines and hair follicles. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in techniques such as Western blotting. By specifically binding to the LGR5 protein, this antibody enables precise detection and analysis in a range of cell types, making it an essential component for studies in stem cell biology and regenerative medicine.
LGR5, also known as leucine-rich repeat-containing G-protein coupled receptor 5, is a key player in stem cell maintenance and tissue regeneration. Its roles in tissue homeostasis and repair make it a promising target for research into therapeutic strategies for conditions such as gastrointestinal disorders and skin diseases. Investigating the functions of LGR5 using this antibody may lead to insights that facilitate the development of innovative treatments that harness the regenerative potential of stem cells.
Product Name:
LGR5/GPR49 Rabbit Polyclonal Antibody
SKU:
CAB10545
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5/GPR49 (NP_003658.1).
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8549
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain using LGR5/GPR49 Rabbit pAb(CAB10545) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
