lexA Antibody (PACO50402)
- SKU:
- PACO50402
- Product Type:
- Antibody
- Reactivity:
- Bacteria - Escherichia coli
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
lexA Antibody (PACO50402)
The LexA Antibody (PAC050402) is a crucial tool for researchers studying the LexA protein, a transcription factor essential for gene regulation in bacteria. This antibody, derived from rabbits, exhibits high reactivity with LexA from various bacterial species and is validated for use in Western blot applications. By binding to the LexA protein, this antibody enables accurate detection and analysis in different bacterial strains, making it ideal for studies in microbiology and genetic research.LexA is a key player in the SOS response pathway, a crucial mechanism for DNA repair in bacteria under genotoxic stress.
Understanding the function of LexA is essential for uncovering the molecular mechanisms behind bacterial DNA repair and survival strategies. Research on LexA is vital for developing antimicrobial strategies and understanding bacterial pathogenicity in various infectious diseases.Get your hands on the LexA Antibody (PAC050402) and advance your research in bacterial genetics and microbiology.
| Antibody Name: | lexA Antibody (PACO50402) |
| Antibody SKU: | PACO50402 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Escherichia coli |
| Immunogen: | Recombinant Escherichia coli LexA repressor protein (1-202AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in Recombinant protein. All lanes: lexA antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. predicted band size: 39 kDa. observed band size: 39 kDa.. |
| Background: | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA. |
| Synonyms: | LexA repressor (EC 3.4.21.88), lexA, exrA spr tsl umuA |
| UniProt Protein Function: | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352). |
| UniProt Protein Details: | |
| NCBI Summary: | LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. [More information is available at EcoGene: EG10533]. LexA is a protein that belongs to the LexA family . [More information is available at EcoCyc: EG10533]. |
| UniProt Code: | P0A7C2 |
| NCBI GenInfo Identifier: | 16131869 |
| NCBI Gene ID: | 948544 |
| NCBI Accession: | NP_418467.1 |
| UniProt Secondary Accession: | P0A7C2,P03033, Q2M6R1 |
| UniProt Related Accession: | P0A7C2 |
| Molecular Weight: | 38.4 kDa |
| NCBI Full Name: | transcriptional repressor of SOS regulon |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | lexAÂ Â |
| NCBI Official Synonym Symbols: | ECK4035; exrA; JW4003; spr; tsl; umuAÂ Â |
| NCBI Protein Information: | transcriptional repressor of SOS regulon |
| UniProt Protein Name: | LexA repressor |
| UniProt Synonym Protein Names: | |
| Protein Family: | LexA repressor |
| UniProt Gene Name: | lexAÂ Â |
| UniProt Entry Name: | LEXA_ECOLI |
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