The Leukosialin Polyclonal Antibody (CAB6412) is a valuable tool for studying leukosialin, also known as CD43, a cell surface glycoprotein involved in immune cell adhesion and activation. This polyclonal antibody, generated in rabbits, is highly specific to human leukosialin and is validated for use in a variety of research applications, including Western blotting.Leukosialin plays a crucial role in regulating immune cell interactions, including T cell activation and trafficking. Its expression has been linked to various immune-related diseases, making it an attractive target for investigation in immunology and cancer research.
By utilizing the CAB6412 antibody, researchers can detect and analyze leukosialin expression in different cell types, aiding in the understanding of its function in both normal and disease states.With the ability to target leukosialin, researchers can gain insights into immune cell behavior and potentially develop new therapeutic strategies for diseases where leukosialin dysregulation is implicated. The Leukosialin Polyclonal Antibody offers a reliable tool for advancing research in immunology and uncovering the role of leukosialin in health and disease.
Product Name:
SPN Rabbit Polyclonal Antibody
SKU:
CAB6412
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 276-400 of human SPN (NP_003114.1).
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome.
Purification Method:
Affinity purification
Gene ID:
6693
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from U-937 cells using SPN Rabbit pAb (CAB6412) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
