The Leptin Receptor Polyclonal Antibody (CAB2999) is a valuable tool for researchers studying the leptin receptor, a protein involved in the regulation of metabolism and energy balance. This antibody is raised in rabbits and has been shown to be highly reactive with human samples, making it ideal for use in Western blot applications.The leptin receptor plays a crucial role in mediating the effects of leptin, a hormone that helps to regulate appetite and body weight. Dysregulation of the leptin receptor has been implicated in obesity and metabolic disorders, making it a target of interest in research on these conditions.
By enabling the detection and analysis of the leptin receptor in various cell types, the CAB2999 antibody can help researchers better understand the mechanisms underlying leptin signaling and its impact on metabolic health. This antibody is a valuable tool for studies in metabolism, obesity, and related research areas.
Product Name:
Leptin Receptor Rabbit Polyclonal Antibody
SKU:
CAB2999
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1070-1165 of human LEPR (NP_002294.2).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
132kDa
Observed MW:
140kDa
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).
Purification Method:
Affinity purification
Gene ID:
3953
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Leptin Receptor antibody (CAB2999) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
