The Leptin Polyclonal Antibody (CAB1300) is a valuable tool for researchers studying the hormone leptin and its roles in metabolism, appetite regulation, and obesity. Leptin, also known as the satiety hormone, plays a crucial role in energy balance by signaling the brain to reduce appetite and increase energy expenditure.Raised in rabbits, this antibody is highly specific and sensitive for detecting leptin in human samples. It has been validated for use in Western blot applications, allowing for the precise detection and quantification of leptin protein levels in various tissues and cell types.
Research on leptin has shown its importance in regulating body weight, metabolism, and insulin sensitivity, making it a key target for understanding and developing treatment strategies for obesity and related metabolic disorders. The Leptin Polyclonal Antibody (CAB1300) can help researchers unravel the complexities of leptin signaling pathways and further our understanding of its implications for human health.
Product Name:
LEP Rabbit Polyclonal Antibody
SKU:
CAB1300
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Leptin (NP_000221.1).
This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development.
Purification Method:
Affinity purification
Gene ID:
3952
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Leptin antibody (CAB1300) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
