The LEPRE1 Polyclonal Antibody (CAB10322) is a valuable tool for researchers studying LEPRE1, a protein crucial for collagen biosynthesis and maintenance of extracellular matrix integrity. This antibody is produced in rabbits and has high specificity for detecting LEPRE1 in human samples, making it suitable for Western blot applications. By binding to the LEPRE1 protein, researchers can gain insights into its role in collagen metabolism and potential implications in diseases such as osteogenesis imperfecta and other connective tissue disorders.
The LEPRE1 Polyclonal Antibody is an essential tool for advancing our understanding of collagen biology and developing targeted therapies for related conditions.
Product Name:
LEPRE1 Rabbit Polyclonal Antibody
SKU:
CAB10322
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 497-736 of human LEPRE1 (NP_071751.3).
This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined.
Purification Method:
Affinity purification
Gene ID:
64175
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using LEPRE1 antibody (CAB10322) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
