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LEPRE1 Rabbit Polyclonal Antibody (CAB10322)

SKU:
CAB10322
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:LEPRE1 Rabbit Polyclonal Antibody
SKU:CAB10322
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 497-736 of human LEPRE1 (NP_071751.3).
Sequence:GDGY RGQT SPHT PNEK FYGV TVFK ALKL GQEG KVPL QSAH LYYN VTEK VRRI MESY FRLD TPLY FSYS HLVC RTAI EEVQ AERK DDSH PVHV DNCI LNAE TLVC VKEP PAYT FRDY SAIL YLNG DFDG GNFY FTEL DAKT VTAE VQPQ CGRA VGFS SGTE NPHG VKAV TRGQ RCAI ALWF TLDP RHSE RDRV QADD LVKM LFSP EEMD LSQE QPLD AQQG PPEP AQES LSGS ESKP KDEL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:2000
Synonyms:OI8; GROS1; LEPRE1
Positive Sample:A375,NCI-H460,HT-29,HeLa,SKOV3
Conjugate:Unconjugated
Cellular Localization:Endoplasmic reticulum, Secreted, extracellular matrix, extracellular space.
Calculated MW:83kDa
Observed MW:100kDa

This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined.

Purification Method:Affinity purification
Gene ID:64175
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using LEPRE1 antibody (CAB10322) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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