The LEMD1 Polyclonal Antibody (PAC061394) is essential for researchers investigating the LEMD1 protein, a key regulator of nuclear envelope dynamics and gene expression. This antibody, produced in rabbits, demonstrates high specificity for human samples and is validated for use in Western blot applications. By binding to the LEMD1 protein, this antibody allows for the detection and analysis of LEMD1 in a variety of cell types, making it an excellent tool for studies in molecular biology and genetics.
LEMD1 is a crucial protein involved in nuclear envelope organization and gene regulation, playing a vital role in maintaining genome stability and cellular function. Dysregulation of LEMD1 has been linked to various human diseases, including muscular dystrophy and cancer, making it an attractive target for further research. Understanding the function and mechanisms of LEMD1 is essential for developing potential therapies targeting these diseases and uncovering new insights into cellular biology.
IHC image of PACO61394 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO61394 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Synonyms:
LEM domain-containing protein 1, Cancer/testis antigen 50, CT50, LEM domain protein 1, LEMP-1, LEMD1
UniProt Protein Function:
LEMD1: 6 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Cancer Testis Antigen (CTA)Chromosomal Location of Human Ortholog: 1q32.1Cellular Component: integral to membrane
