The LEF1 Rabbit Monoclonal Antibody (CAB4473) is a highly specific antibody designed for research involving LEF1, a transcription factor that plays a key role in the Wnt signaling pathway and regulation of gene expression. This antibody, generated in rabbits, has been validated for use in immunohistochemistry, immunofluorescence, and other application methods. It targets the LEF1 protein, allowing for precise detection and analysis in a variety of cell types and tissues.LEF1 is a crucial factor in developmental processes, cell differentiation, and tissue homeostasis.
Dysregulation of LEF1 has been implicated in various diseases, including cancer and inflammatory disorders. By studying the activity of LEF1, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and identify potential therapeutic targets. The LEF1 Rabbit Monoclonal Antibody is a powerful tool for investigating the function and expression of LEF1, making it an essential reagent for studies in developmental biology, oncology, and beyond.
Product Name:
LEF1 Rabbit Monoclonal Antibody
SKU:
CAB4473
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant protein fo a sequence within amino acids 50-150 of human LEF1 (Q9UJU2).
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
51176
Clone Number:
ARC1019
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using LEF1 Rabbit mAb (CAB4473) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
