The LEF1 Polyclonal Antibody (CAB0909) is a crucial tool for researchers studying the LEF1 protein, a key transcription factor involved in the Wnt signaling pathway. This antibody, produced in rabbits, shows high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to LEF1, researchers can accurately detect and analyze the protein in various cell types, making it an essential reagent for studies in developmental biology, cancer research, and stem cell biology.
LEF1 plays a vital role in regulating gene expression and cell fate determination during embryonic development and tissue regeneration. Dysregulation of the Wnt signaling pathway, in which LEF1 is a key player, has been implicated in various diseases, including cancer and developmental disorders. By understanding the function of LEF1, researchers can gain insights into the underlying mechanisms of these diseases and potentially develop targeted therapies to treat them.
Product Name:
LEF1 Rabbit Polyclonal Antibody
SKU:
CAB0909
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-132 of human LEF1 (NP_057353.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIF/ICC,1:50 - 1:200
Synonyms:
LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF1
Positive Sample:
BxPC-3,Jurkat,Mouse thymus,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
25-58kDa
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
51176
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using LEF1 Rabbit pAb (CAB0909) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
