The LDLR Polyclonal Antibody (CAB14996) is a valuable tool for researchers studying the low-density lipoprotein receptor (LDLR), a crucial protein involved in cholesterol metabolism and cardiovascular health. This antibody, produced in rabbits, shows high reactivity with LDLR in human samples and is validated for Western blot applications.LDLR plays a key role in the regulation of cholesterol levels in the blood by facilitating the uptake of LDL particles. Dysregulation of LDLR has been linked to a variety of cardiovascular diseases, including atherosclerosis and familial hypercholesterolemia.
By targeting LDLR with this antibody, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for cardiovascular disorders.In addition to its role in cholesterol metabolism, LDLR has also been implicated in other physiological processes such as cell signaling and immune response modulation. The LDLR Polyclonal Antibody provides a valuable tool for investigating these diverse functions, making it an essential reagent for studies in cardiovascular biology, lipid metabolism, and related fields.
Product Name:
LDLR Rabbit Polyclonal Antibody
SKU:
CAB14996
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Cell membrane, Cell surface, Early endosome, Endomembrane system, Golgi apparatus, Late endosome, Lysosome, Membrane, Single-pass type I membrane protein, clathrin-coated pit.
Calculated MW:
95kDa
Observed MW:
100-160kDa
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3949
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LDL Receptor (LDLR) Rabbit pAb (CAB14996) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
