LDHB Recombinant Protein (RPPB1898)
- SKU:
- RPPB1898
- Product Type:
- Recombinant Protein
- Research Area:
- Enzymes
Frequently bought together:
Description
Product Name: | LDHB Recombinant Protein |
Product Code: | RPPB1898 |
Size: | 3mg |
Target: | LDHB |
Synonyms: | Lactate Dehydrogenase, LDH. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile lyophilized powder. |
Formulation: | The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate. |
Solubility: | It is recommended to reconstitute the lyophilized LDH in sterile 18M?-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions. |
Stability: | Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Biological Activity: | The specific activity was found to be 258 U/mg protein. |
Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.