Anti-LDHA Antibody (CAB16394)
- SKU:
- CAB16394
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
Anti-LDHA Antibody (CAB16394)
The LDHA Polyclonal Antibody (CAB16394) is a valuable tool for researchers studying lactate dehydrogenase A (LDHA), a key enzyme involved in the conversion of lactate to pyruvate. LDHA plays a crucial role in cellular metabolism, particularly in cancer cells where it is often upregulated to support rapid growth and proliferation.Raised in rabbits, this antibody is highly specific to human LDHA and has been validated for use in Western blot applications. By targeting the LDHA enzyme, researchers can detect and analyze its expression levels in various cell types, providing insights into metabolic processes and potential therapeutic targets in cancers and other metabolic diseases.
The LDHA Polyclonal Antibody is an essential tool for studying the role of LDHA in cancer metabolism and developing new strategies for targeted therapies. Its high reactivity and specificity make it an ideal choice for researchers in the fields of oncology and metabolism.
| Antibody Name: | Anti-LDHA Antibody |
| Antibody SKU: | CAB16394 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human LDHA |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, COS-7, HepG2, A-431, Mouse liver, Rat liver |
| Immunogen: | Recombinant protein of human LDHA |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3939 |
| Uniprot: | P00338 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 26kDa/30kDa/36kDa/39kDa |
| Observed MW: | 37kDa |
| Synonyms: | GSD11, HEL-S-133P, LDHM, PIG19, LDHA |
| Background: | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. |
| NCBI Summary: | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P00338 |
| NCBI GenInfo Identifier: | 126047 |
| NCBI Gene ID: | 3939 |
| NCBI Accession: | P00338.2 |
| UniProt Secondary Accession: | P00338,Q53G53, Q6IBM7, Q6ZNV1, Q9UDE8, Q9UDE9, B4DKQ2 B7Z5E3, D3DQY3, F8W819, |
| UniProt Related Accession: | P00338 |
| Molecular Weight: | 26,712 Da |
| NCBI Full Name: | L-lactate dehydrogenase A chain |
| NCBI Synonym Full Names: | lactate dehydrogenase A |
| NCBI Official Symbol: | LDHAÂ Â |
| NCBI Official Synonym Symbols: | LDHM; GSD11; PIG19; HEL-S-133PÂ Â |
| NCBI Protein Information: | L-lactate dehydrogenase A chain |
| UniProt Protein Name: | L-lactate dehydrogenase A chain |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59 |
| Protein Family: | L-lactate dehydrogenase |
| UniProt Gene Name: | LDHAÂ Â |
| UniProt Entry Name: | LDHA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LDHA antibody (CAB16394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using LDHA antibody (CAB16394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using LDHA Rabbit pAb (CAB16394) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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