The LDHA Polyclonal Antibody (CAB1146) is a valuable tool for researchers studying lactate dehydrogenase A (LDHA), an enzyme that plays a crucial role in energy production and metabolism in cells. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to LDHA, this antibody enables accurate detection and analysis of the enzyme in various cell types, making it an ideal choice for studies in metabolism, cancer research, and drug development.
LDHA is a key player in the Warburg effect, a metabolic phenotype commonly observed in cancer cells where they rely on glycolysis for energy production even in the presence of oxygen. Targeting LDHA has emerged as a promising strategy for developing novel anticancer therapies. This antibody provides researchers with a reliable tool for investigating the role of LDHA in cancer progression and metabolic reprogramming, opening up new avenues for therapeutic interventions.
Product Name:
LDHA Rabbit Polyclonal Antibody
SKU:
CAB1146
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Synonyms:
LDHM; GSD11; PIG19; HEL-S-133P; LDHA
Positive Sample:
293T,Rat liver,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
37kDa
Observed MW:
37kDa
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Purification Method:
Affinity purification
Gene ID:
3939
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver using LDHA Rabbit pAb(CAB1146) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
![[KD Validated] LDHA Polyclonal Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/223969/586868/kd-validated-ldha-polyclonal-antibody__81801.1701189112.jpg?c=2 "[KD Validated] LDHA Polyclonal Antibody")
