The LDHA Monoclonal Antibody is a valuable tool for researchers studying lactate dehydrogenase A (LDHA), an enzyme involved in the conversion of lactate to pyruvate in the glycolysis pathway. This antibody, produced using monoclonal technology, specifically targets LDHA and is highly reactive with human samples. Validated for use in various applications such as Western blotting and immunohistochemistry, this antibody enables the detection and analysis of LDHA expression in different cell types. Its versatility and specificity make it an ideal choice for studies focused on metabolism, cancer research, and other related fields.
LDHA is a key player in cellular metabolism, with dysregulation of its activity implicated in various diseases, including cancer. By studying the expression and function of LDHA using this monoclonal antibody, researchers can gain insights into the role of this enzyme in health and disease, potentially leading to the development of novel therapeutic strategies targeting LDHA.
Product Name:
LDHA Monoclonal Antibody
SKU:
CAB21893
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 269-283 of human LDHA (NP_005557.1).
Sequence:
RVHP VSTM IKGL YGI
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
LDHM; GSD11; PIG19; HEL-S-133P; LDHA
Positive Sample:
HeLa,293T,MCF7,Mouse liver,Rat lung,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
37kDa
Observed MW:
Refer to figures
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Purification Method:
Affinity purification
Gene ID:
3939
Clone Number:
ARC0509
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using LDHA Rabbit mAb (CAB21893) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
![[KD Validated] LDHA Polyclonal Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/223969/586868/kd-validated-ldha-polyclonal-antibody__81801.1701189112.jpg?c=2 "[KD Validated] LDHA Polyclonal Antibody")
