The LCN2 Polyclonal Antibody (CAB3176) is a valuable tool for researchers studying Lipocalin 2 (LCN2), a protein involved in a variety of biological processes such as cell growth, differentiation, and immune responses. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the LCN2 protein, this antibody enables researchers to detect and analyze LCN2 levels in different cell types, making it well-suited for studies in immunology, cancer research, and other related fields.
LCN2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a key player in the regulation of inflammatory responses and is implicated in various diseases such as cancer, kidney injury, and inflammatory disorders. By understanding the role of LCN2 in these pathological conditions, researchers can potentially develop targeted therapies that modulate its activity for therapeutic benefit. The LCN2 Polyclonal Antibody is a valuable tool for investigating the function of LCN2 and its potential as a target for therapeutic intervention.
Product Name:
Lipocalin-2/NGAL Rabbit Polyclonal Antibody
SKU:
CAB3176
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lipocalin-2/NGAL (NP_005555.2).
Sequence:
SKVP LQQN FQDN QFQG K
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
p25; 24p3; MSFI; NGAL; Lipocalin-2/NGAL
Positive Sample:
293F(negative),A-431
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
23kDa
Observed MW:
22kDa
This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice.
Purification Method:
Affinity purification
Gene ID:
3934
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Lipocalin-2/NGAL antibody (CAB3176) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
