The LCN2 Polyclonal Antibody (PACO64291) is a valuable tool for researchers studying Lipocalin-2 (LCN2), a protein involved in various physiological processes, including immune response and iron transport. This antibody, produced in rabbits, offers high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.LCN2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a key player in inflammation and innate immunity, making it a promising target for studies in immunology, cancer research, and metabolic disorders.
By targeting LCN2 with this antibody, researchers can investigate its role in disease pathogenesis and potential therapeutic interventions.With its ability to detect and analyze LCN2 in different cell types and tissues, this polyclonal antibody is a versatile tool for exploring the functions of this protein in various biological contexts. By understanding the mechanisms underlying LCN2 activity, researchers can gain insights into its potential as a biomarker or therapeutic target in diseases characterized by dysregulated immune responses and iron metabolism.
IHC image of PACO64291 diluted at 1:200 and staining in paraffin-embedded rat lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Background:
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (By similarity). Binds iron through association with 2,5-dihydroxybenzoic acid, (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis (By similarity).
LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Transporter; Apoptosis; Secreted, signal peptide; Secreted
Molecular Function:protein homodimerization activity; protease binding; transporter activity; iron ion binding
Biological Process: response to drug; response to nutrient levels; response to mycotoxin; response to virus; positive regulation of cell projection organization and biogenesis; response to herbicide; cellular response to nutrient levels; iron ion homeostasis; response to bacterium; siderophore transport; innate immune response; ion transport; response to oxidative stress
NCBI Summary:
mouse homolog plays a role in IL3 withdrawal-induced apoptosis [RGD, Feb 2006]
