Based on the information from the provided URL, the LCMT1 Polyclonal Antibody (PACO16608) is a cutting-edge tool for researchers studying LCMT1, a key protein involved in cellular processes such as protein methylation. This antibody, developed and tested by Assay Genie, specifically targets LCMT1 and is highly reactive with human samples, making it a reliable choice for various applications in biochemical and biomedical research.LCMT1 is a critical enzyme involved in the post-translational modification of proteins, particularly in the methylation process that influences their functions. By targeting LCMT1 with this polyclonal antibody, researchers can detect and analyze the expression and activity of this protein in different cell types, providing insights into its role in cellular signaling pathways and disease mechanisms.
Moreover, the LCMT1 Polyclonal Antibody offers high specificity and sensitivity in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, allowing for precise detection and visualization of LCMT1 in experimental settings. Researchers studying protein methylation, cell signaling, and related pathways will find this antibody invaluable for their investigations.Overall, the LCMT1 Polyclonal Antibody (PACO16608) from Assay Genie represents a valuable tool for advancing research in molecular biology, biochemistry, and drug discovery, providing a deeper understanding of the functions and potential therapeutic implications of LCMT1 in various physiological and pathological conditions.
Antibody Name:
LCMT1 Antibody (PACO16608)
Antibody SKU:
PACO16608
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human, Rat
Immunogen:
Fusion protein of human LCMT1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO16608(LCMT1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO16608(LCMT1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A.
Synonyms:
leucine carboxyl methyltransferase 1
UniProt Protein Function:
LCMT1: Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. Belongs to the methyltransferase superfamily. LCMT family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - histidine; EC 2.1.1.233; Lipid Metabolism - androgen and estrogen; Methyltransferase; Other Amino Acids Metabolism - selenoamino acidChromosomal Location of Human Ortholog: 16p12.1Cellular Component: cytosolMolecular Function: protein binding; C-terminal protein carboxyl methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activityBiological Process: regulation of apoptosis; protein amino acid methylation; C-terminal protein amino acid methylation; protein modification process; negative regulation of protein complex assembly
UniProt Protein Details:
NCBI Summary:
LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]
