The Lck Polyclonal Antibody (CAB2177) is a valuable tool for researchers studying Lck, a protein tyrosine kinase involved in T cell receptor signaling. This antibody, raised in rabbits, is highly specific to human Lck and has been validated for use in Western blot applications. It binds to the Lck protein, allowing for the detection and analysis of Lck in various cell types, making it ideal for studies in immunology and cancer research.
Lck plays a crucial role in the activation of T cells and is essential for T cell-mediated immune responses. Dysregulation of Lck activity has been implicated in various immune-related disorders, making it a promising target for therapeutic interventions. By understanding the function of Lck, researchers can gain insights into immune regulation and potentially develop novel treatments for immune-related diseases.
Product Name:
LCK Rabbit Polyclonal Antibody
SKU:
CAB2177
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LCK (NP_005347.3).
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
3932
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse thymus, using LCK Rabbit pAb (CAB2177) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
