The LCAT Polyclonal Antibody (CAB11745) is a vital tool for researchers studying Lecithin-Cholesterol Acyltransferase (LCAT), an enzyme involved in the metabolism of cholesterol. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the LCAT protein, researchers can accurately detect and analyze this enzyme in various cell types, making it an essential component in studies related to lipid metabolism, cardiovascular diseases, and kidney function.LCAT plays a crucial role in the formation of high-density lipoprotein (HDL) particles, known as the "good cholesterol," by catalyzing the esterification of free cholesterol.
Dysregulation of LCAT activity has been linked to dyslipidemia, atherosclerosis, and other lipid-related disorders, highlighting the importance of studying this enzyme in the context of cardiovascular health. The LCAT Polyclonal Antibody provides researchers with a reliable tool to investigate the function and regulation of LCAT, paving the way for new insights into lipid metabolism and potential therapeutic targets for related diseases.
Product Name:
LCAT Rabbit Polyclonal Antibody
SKU:
CAB11745
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-440 of human LCAT (NP_000220.1).
This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency.
Purification Method:
Affinity purification
Gene ID:
3931
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Human plasma, using LCAT Rabbit pAb (CAB11745) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
