LATS1 Rabbit Polyclonal Antibody (CAB17992)
- SKU:
- CAB17992
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
Product Name: | LATS1 Rabbit Polyclonal Antibody |
SKU: | CAB17992 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human LATS1 (NP_004681.1). |
Sequence: | EVNP QMLQ DLQA AGFD EDMV IQAL QKTN NRSI EAAI EFIS KMSY QDPR REQM AAAA ARPI NASM KPGN VQQS VNRK QSWK GSKE SLVP QRHG PPLG ESVA Y |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 |
Synonyms: | wts; WARTS; LATS1 |
Positive Sample: | HeLa,C2C12 |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center. |
Calculated MW: | 127kDa |
Observed MW: | 150kDa |
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Purification Method: | Affinity purification |
Gene ID: | 9113 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |