The Phospho-LATS1 (S909) / LATS2 (S872) Polyclonal Antibody (CABP0904) is a valuable tool for researchers studying the Hippo signaling pathway. This antibody, raised in rabbits, specifically detects phosphorylation of LATS1 at Serine 909 and LATS2 at Serine 872, key regulatory sites in the pathway. The Hippo pathway plays a crucial role in controlling organ size, tissue homeostasis, and tumor suppression. Phosphorylation of LATS1 and LATS2 is essential for their activation and downstream signaling, ultimately leading to regulation of cell proliferation and apoptosis.
This antibody is suitable for use in Western blot applications and is highly reactive with human samples. Its specificity for phosphorylated LATS1 and LATS2 makes it an ideal tool for investigating the role of the Hippo pathway in various cell types and disease conditions, particularly in cancer research and regenerative medicine studies. Understanding the activation and regulation of LATS1 and LATS2 through phosphorylation is essential for uncovering the therapeutic potential of targeting the Hippo pathway in disease treatment strategies.
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Purification Method:
Affinity purification
Gene ID:
9113/26524
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HeLa, using phospho-LATS1-S909/LATS2-S872 Rabbit pAb (CABP0904) at 1:500 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
